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Endogenous tissue factor pathway inhibitor in vascular smooth muscle cells inhibits arterial thrombosis

Jichun Yang, Kaiyue Jin, Jiajun Xiao, Jing Ma, Duan Ma

Frontiers of Medicine 2017, Volume 11, Issue 3,   Pages 403-409 doi: 10.1007/s11684-017-0522-y

Abstract: In this TFPIFlox mice crossbred with Sma–Cre mice were utilized to establish TFPIconditional knockout mice and to examine the effects of VSMC-directed TFPI deletion on development,The mice with deleted TFPI in VSMCs (TFPISma) reproduced viable offspring.cells and cells of hematopoietic origin) mice.that of the TFPIFlox mice.

Keywords： arterial thrombosis     conditional knockout mice     tissue factor pathway inhibitor     vascular smooth muscle    

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Named entity recognition for Chinese construction documents based on conditional random field

Frontiers of Engineering Management 2023, Volume 10, Issue 2,   Pages 237-249 doi: 10.1007/s42524-021-0179-8

Abstract: This study presents a NER method for Chinese construction documents based on conditional random field

Keywords： NER     NLP     Chinese language     construction document    

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Effect of PRAK gene knockout on the proliferation of mouse embryonic fibroblasts

Xiaowei GONG MD, PhD, Xiaoyan MING MD, Xu WANG MM, Daan WANG MD, Peng DENG MM, Yong JIANG MD, PhD, Aihua LIU MD, PhD,

Frontiers of Medicine 2009, Volume 3, Issue 4,   Pages 379-383 doi: 10.1007/s11684-009-0073-y

Abstract: Taken together, gene knockout in MEF cells leads to cell cycle arrest and proliferation inhibition.

Keywords： p38 regulated/activated protein kinase     gene knockout     cell cycle     cell proliferation    

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Coupling the porous conditional moment closure with the random pore model: applications to gasification

D. N. SAULOV, C. R. CHODANKA, M. J. CLEARY, A. Y. KLIMENKO

Frontiers of Chemical Science and Engineering 2012, Volume 6, Issue 1,   Pages 84-93 doi: 10.1007/s11705-011-1164-2

Abstract: The porous conditional moment closure (PCMC) model proves to be adequate for modeling reactive transport

Keywords： gasification     CO2 capture     PCMC     RPM    

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Carbon dots-based fluorescence sensor for two-photon imaging of pH in diabetic mice

Frontiers of Chemical Science and Engineering 2023, Volume 17, Issue 3,   Pages 298-306 doi: 10.1007/s11705-022-2212-9

Abstract: CA-CDs has been successfully applied for two-photon imaging of the pH in liver and kidney of diabetic miceImaging results showed that the pH value in kidney of diabetic mice was lower than that of the normalmice, while the pH value in liver of diabetic mice was almost the same as that of the normal mice.

Keywords： carbon dots     two-photon imaging     pH     diabetic mice    

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Metabonomic study of the biochemical profiles of heterozygous myostatin knockout swine

Jianxiang XU,Dengke PAN,Jie ZHAO,Jianwu WANG,Xiaohong HE,Yuehui MA,Ning LI

Frontiers of Agricultural Science and Engineering 2015, Volume 2, Issue 1,   Pages 90-99 doi: 10.15302/J-FASE-2015045

Abstract: Myostatin gene ( ) knockout (KO) mice show possible effects for the prevention or treatment of metabolic

Keywords： myostatin     transforming growth factor-β family     skeletal muscle     metabolic disorders     chromatography     mass spectrometry     metabonomics    

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Inhibitory activity of Bifidobacterium adolescent combined with cisplatin on melanoma in mice and its

HUANG Hongying, LIU Guangchao, QI Yijun, DU Yaowu, CHEN Jugao, MA Yuanfang

Frontiers of Medicine 2008, Volume 2, Issue 2,   Pages 186-190 doi: 10.1007/s11684-008-0035-9

Abstract: inhibitory activity of Bifidobacterium adolescent combined with cisplatin on the growth of melanoma (B16) in miceC57 mice were inoculated with B16 cancer cells to construct mouse model of melanoma and treated withThe ratio of inhibitory activity reached 74.45% when the mice were treated by bifidobacterium combinedthe inhibitory activity on B16 cell proliferation is correlated with the enhanced immune activity in mice
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Particulate matter 2.5 triggers airway inflammation and bronchial hyperresponsiveness in mice by activating

Frontiers of Medicine 2021, Volume 15, Issue 5,   Pages 750-766 doi: 10.1007/s11684-021-0839-4

Abstract: and acetylation, airway inflammation, and bronchial hyperresponsiveness were deteriorated in SIRT2 knockoutmice exposed to PM2.5.

Keywords： particulate matter 2.5     sirtuin 2     p65     airway inflammation     bronchial hyperresponsiveness     triptolide    

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Immunogenicity and protective immunity against otitis media caused by pneumococcus in mice of Hib conjugate

Zeyu Chen,Rong Guo,Jianghong Xu,Chuangjun Qiu

Frontiers of Medicine 2016, Volume 10, Issue 4,   Pages 490-498 doi: 10.1007/s11684-016-0470-y

Abstract: polysaccharide conjugate vaccine with the pneumococcal surface adhesin A (PsaA) protein carrier in young miceA total of 15 young mice aged 3 weeks to 5 weeks were immunized with the conjugate vaccine, and another15 young mice of the same age were immunized with the licensed Hib-tetanus toxoid (TT) vaccine.Furthermore, the third group of 15 young mice was inoculated with phosphate buffer saline as control.The immunized mice were inoculated with pneumococcus in the middle ear.

Keywords： conjugate vaccine     pneumococcal surface adhesin A     Hemophilus influenzae b     immunogenicity     otitis media    

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Gene therapy for hemophilia B mice with scAAV8-LP1-hFIX

Wei Lu,Qingzhang Zhou,Hao Yang,Hao Wang,Yexing Gu,Qi Shen,Jinglun Xue,Xiaoyan Dong,Jinzhong Chen

Frontiers of Medicine 2016, Volume 10, Issue 2,   Pages 212-218 doi: 10.1007/s11684-016-0438-y

Abstract:

Hemophilia B is a hemorrhagic disease caused by the deficiency of clotting factor IX (FIX). Gene therapy might be the ultimate strategy for the disease. However, two main problems that should be solved in gene therapy for hemophilia B are immunity and safety. Self-complementary adeno-associated virus serotype 8 (scAAV8), a non-human primate AAV featuring low immunogenicity and high transfection efficiency in liver cells, might be a potential vector for hemophilia B gene therapy. A strong liver-specific promoter-1 (LP1) was inserted and mutant human FIX Arg338Ala was introduced into plasmid scAAV8-LP1 to develop an optimized AAV8 vector that expresses human clotting factor FIX (hFIX). The efficiency of scAAV8-LP1-hFIX administered through normal systemic injection or hydrodynamic injection was compared. A high expression was achieved using hydrodynamic injection, and the peak hFIX expression levels in the 5×1011 and 1×1011 virus genome (vg) cohorts were 31.94% and 25.02% of normal level, respectively, at 60 days post-injection. From the perspective of long-term (200 days) expression, both injection methods presented promising results with the concentration value maintained above 4% of normal plasma. The results were further verified by enzyme-linked immunosorbent assay and activated partial thromboplastin time. Our study provides a potential gene therapy method for hemophilia B.

Keywords： hemophilia B     AAV8     hFIX     gene therapy    

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Overexpression of netrin-1 improves neurological outcomes in mice following transient middle cerebral

Haiyan LU, Yongting WANG, Falei YUAN, Jianrong LIU, Lili ZENG, Guo-Yuan YANG

Frontiers of Medicine 2011, Volume 5, Issue 1,   Pages 86-93 doi: 10.1007/s11684-011-0118-x

Abstract: The level of NT-1 was increased in the AAV-NT-1-transduced tMCAO mice compared with the control mice.Furthermore, the neurobehavioral outcomes were significantly improved in AAV-NT-1-transduced mice compared

Keywords： adeno-associated virus     angiogenesis     gene transfer     ischemia     middle cerebral artery occlusion     netrin-1    

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One-step generation of myostatin gene knockout sheep via the CRISPR/Cas9 system

Hongbing HAN,Yonghe MA,Tao WANG,Ling LIAN,Xiuzhi TIAN,Rui HU,Shoulong DENG,Kongpan LI,Feng WANG,Ning LI,Guoshi LIU,Yaofeng ZHAO,Zhengxing LIAN

Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 1,   Pages 2-5 doi: 10.15302/J-FASE-2014007

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Long-term correction of hemorrhagic diathesis in hemophilia A mice by an AAV-delivered hybrid FVIII composed

Frontiers of Medicine 2022, Volume 16, Issue 4,   Pages 584-595 doi: 10.1007/s11684-021-0844-7

Abstract: (hLC), and rat light chain (rLC) were transfected into cells and hydrodynamically injected into HA micePurified AAV viruses were intravenously injected into HA mice at two doses.Notably, the HA mice undergoing the AAV-delivered hHC+ rLC treatment exhibited a visibly higher activity+ rLC) via an AAV-delivered gene therapy substantially improved the hemorrhagic diathesis of the HA mice

Keywords： hemophilia A     adeno-associated virus (AAV)     human/rat hybrid factor VIII     gene therapy     dual chain strategy    

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The Model on Maximum Entropy of Knowledge Creating Stochastic Process

Chen Xin,He Jinsheng,Dong Liping

Strategic Study of CAE 2004, Volume 6, Issue 12,   Pages 43-46

Abstract: theory, the optimization theory and the maximum entropy principle comprehensively, and puts forward the conditional

Keywords： maximum entropy     knowledge innovation     conditional entropy     stochastic process    

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Development of the expressed immunoglobulin μ chain repertoire during maturation of mice B cells

Jingwen LIANG,Yingfeng LUO,Yi SUN,Meng LEI,Bing ZHANG,Songnian HU,Yaofeng ZHAO

Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 3,   Pages 201-213 doi: 10.15302/J-FASE-2014017

Abstract: In the bone marrow and spleen, the developing B cell populations undergo both negative and positive selections to shape their B cell receptor repertoire. To gain insight into the shift of the immunoglobulin heavy (IgH) chain repertoire during B cell development, we undertook large scale Ig μ chain repertoire analysis of pre-B, immature B and spleen B cell populations. We found that the majority of V gene segments, V families, J and D gene segments, were observed to have significantly different usage frequencies when three B cell populations were compared, but the usage profile of the V D, and J genes between different B cell populations showed high correlations. In both productive and nonproductive rearrangements, the length of CDRH3 shortened significantly on average when B cells entered the periphery. However, the CDRH3 length distribution of nonproductive rearrangements did not follow a Gaussian distribution, but decreased successively in the order 3 -2, 3 -1 and 3 , suggesting a direct correlation between mRNA stability and CDRH3 length patterns of nonproductive rearrangements. Further analysis of the individual components comprising CDRH3 of productive rearrangements indicated that the decrease in CDRH3 length was largely due to the reduction of N addition at the 5′ and 3′ junctions. Moreover, with development, the amino acid content of CDRH3 progressed toward fewer positively charged and nonpolar residues but more polar residues. All these data indicated that the expressed Ig μ chain repertoire, especially the repertoire of CDRH3, was fine-tuned when B cells passed through several checkpoints of selection during the process of maturation.

Keywords： repertoire     complementarity determining region 3 of the IgH chain (CDRH3)     immunoglobulin     heavy chain     variable region    

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Title Author Date Type Operation

Endogenous tissue factor pathway inhibitor in vascular smooth muscle cells inhibits arterial thrombosis

Jichun Yang, Kaiyue Jin, Jiajun Xiao, Jing Ma, Duan Ma

Journal Article

Named entity recognition for Chinese construction documents based on conditional random field

Journal Article

Effect of PRAK gene knockout on the proliferation of mouse embryonic fibroblasts

Xiaowei GONG MD, PhD, Xiaoyan MING MD, Xu WANG MM, Daan WANG MD, Peng DENG MM, Yong JIANG MD, PhD, Aihua LIU MD, PhD,

Journal Article

Coupling the porous conditional moment closure with the random pore model: applications to gasification

D. N. SAULOV, C. R. CHODANKA, M. J. CLEARY, A. Y. KLIMENKO

Journal Article

Carbon dots-based fluorescence sensor for two-photon imaging of pH in diabetic mice

Journal Article

Metabonomic study of the biochemical profiles of heterozygous myostatin knockout swine

Jianxiang XU,Dengke PAN,Jie ZHAO,Jianwu WANG,Xiaohong HE,Yuehui MA,Ning LI

Journal Article

Inhibitory activity of Bifidobacterium adolescent combined with cisplatin on melanoma in mice and its

HUANG Hongying, LIU Guangchao, QI Yijun, DU Yaowu, CHEN Jugao, MA Yuanfang

Journal Article

Particulate matter 2.5 triggers airway inflammation and bronchial hyperresponsiveness in mice by activating

Journal Article

Immunogenicity and protective immunity against otitis media caused by pneumococcus in mice of Hib conjugate

Zeyu Chen,Rong Guo,Jianghong Xu,Chuangjun Qiu

Journal Article

Gene therapy for hemophilia B mice with scAAV8-LP1-hFIX

Wei Lu,Qingzhang Zhou,Hao Yang,Hao Wang,Yexing Gu,Qi Shen,Jinglun Xue,Xiaoyan Dong,Jinzhong Chen

Journal Article

Overexpression of netrin-1 improves neurological outcomes in mice following transient middle cerebral

Haiyan LU, Yongting WANG, Falei YUAN, Jianrong LIU, Lili ZENG, Guo-Yuan YANG

Journal Article

One-step generation of myostatin gene knockout sheep via the CRISPR/Cas9 system

Hongbing HAN,Yonghe MA,Tao WANG,Ling LIAN,Xiuzhi TIAN,Rui HU,Shoulong DENG,Kongpan LI,Feng WANG,Ning LI,Guoshi LIU,Yaofeng ZHAO,Zhengxing LIAN

Journal Article

Long-term correction of hemorrhagic diathesis in hemophilia A mice by an AAV-delivered hybrid FVIII composed

Journal Article

The Model on Maximum Entropy of Knowledge Creating Stochastic Process

Chen Xin,He Jinsheng,Dong Liping

Journal Article

Development of the expressed immunoglobulin μ chain repertoire during maturation of mice B cells

Jingwen LIANG,Yingfeng LUO,Yi SUN,Meng LEI,Bing ZHANG,Songnian HU,Yaofeng ZHAO

Journal Article